• Great fun in working with plants

    Adventure in Plant Science through Faith




    我們歡迎有學習熱情 能跑 能跳 能思考 能抗壓的大專生加入主題計畫!

  • Education and Academic Life

    Expertise: Plant molecular and cell biology; Plant physiology; Plant molecular transport; Plant biotechnology

    2019 - present Associate Professor, Institute of Bioinformatics & Structural Biology, NTHU

    2014 - 2019 Assistant Professor, Institute of Bioinformatics & Structural Biology, National Tsing Hua University (NTHU)

    2008 - 2014 Postdoc, Agricultural Biotechnology Research Center (ABRC), Academia Sinica

    2008 Ph.D. Center for Plant Molecular Biology (ZMBP), Department of Plant Physiology, Universität Tübingen, Germany

    2003 M.S. Institute of Microbiology & Immunology, NYMU

    2001 B.S. Department of Biotechnology & Laboratory Science in Medicine, National Yang-Ming University (NYMU)

  • Research Interests

    Plants as autotrophic organisms capture and convert the solar energy, and store or distribute it in various forms of biomolecules to provide us food, medicine, and materials. As their health and productivity impose a great impact on nearly every aspect of human activities, plant science becomes an important and fascinating research field in perspective of achieving sustainability in agriculture and environment. In our lab, we are interested in identifying molecular mechanisms by which plants respond and adapt to a variety of abiotic stresses as well as developing tools or techniques to address fundamental questions in plant biology through innovation. Our research is currently focused on the following topics using Arabidopsis and Nicotiana benthamiana, a close relative of tobacco, as model organisms:

    • Crosstalk of plant autophagy and phosphate (Pi) homeostasis

    Nutrient starvation-induced autophagy is conserved among eukaryotic organisms including higher plants. Although plant autophagy-deficient mutants are not lethal, they display early leaf senescence and reduced seed yield, and exhibit hypersensitivity to various stresses. Compared to the wealth of investigations on carbon (C) and nitrogen (N) starvation-induced autophagy, our understanding of the role of autophagy in the maintenance of plant phosphate (Pi) homeostasis and plant adaptation to Pi starvation remains relatively limited. We're currently addressing these key questions: 1) What role does autophagy play in regulation of Pi homeostasis? 2) By which mechanism do plant cells sense Pi limitation and specifically induce the selective autophagy for internal Pi recycling?

    Dual-intein-coupled tripartite split-GFP sensor for membrane PPI
    • Developing molecular tools for protein-protein interaction screening in planta

    Searching for novel protein-protein interactions becomes a major and challenging task to elucidate protein functions as well as its regulatory network. Although in planta screening methods, which allow for proper protein modifications and physiologically relevant interaction environment, are potentially more reliable in terms of minimizing unspecific behaviors observed in heterologous systems, only few attempts have been made for establishing library-scale screens in planta. Our research project is aimed to develop a new molecular tool for high-throughput screens of protein-protein interactions in planta, particularly suitable for identification of transporter-interacting proteins.

    • Improving the performance of Plant Microbial Fuel Cell (PMFC) and MFC as clean energy source

    Plant Microbial fuel cell (PMFC) and MFC are sustainable technologies that can produce electrons using plants and microbes. However, low power density and high cost are the two major issues that hamper their development. New design and materials used to build the electrodes as well as the genetic engineering of living organisms are of great potential to enhance the electron transfer. We're currently collaborating with Prof. Han-Yi Chen’s team at Department of Materials Science and Engineering, NTHU, for inventing self-powered devices

  • Awards and honors

    2001 National Certificate of Qualification of Laboratory Science in Medicine (國家醫檢師執照)

    2004 – 2005 Deutsche Forschungsgemeinschaft (DFG) Promotionsstipendium

    2006 – 2008 Wissenschaftliche Mitarbeiter (zur Promotion an Tübingen Universität)

    2009 – 2012 Academia Sinica Postdoctoral Fellowship

    2011 Young Scientist Award Second Prize (Plant Section), the Society for Experimental Biology

    2012 國科會博士後研究人員學術著作獎

    2013 Oct Technical Training - vacuole isolation and transport assay in Prof. Enrico Martinoia’s lab, Institute of Plant Biology, University of Zürich, Switzerland

    2014 中華植物學會暨創新植物及生物科技研討會 講員

    2016 第九屆楊祥發院士傑出農業科學年輕學者獎

    2017 國立清華大學第三十屆新進人員研究獎

    2017 台灣生物化學及分子生物學學會/生化秋令營 講員

    2018 台灣植物學會新秀獎

    2018 台灣植物學會/植物分子夏令營 講員

    2019 日台植物生物學國際研討會 海報/口頭演講

    2019 Advance in Biophysics and Beyond Meeting 講員 (One-day Satellite Meeting of the Biophysics Society Conference)

    2019 Integrative Organismal Biology Symposium 講員

    2019 國立清華大學第十ㄧ屆傑出導師獎

  • Publications (2008-2022)


    1. Lin, F.-Y., Lin, Y.-Y., Li, H.-T., Ni, C.-S., Liu, C.-I., Guan, C.,-Y., Chang, C.-C., Yu, C.-P., Chen, W.-S., Liu, T.-Y.#, Chen, H.-Y.# (2022) Trapa natans husk-derived carbon as a sustainable electrode material for plant microbial fuel cells, Applied Energy 325, 119807. (#corresponding author; SCI; 2021 IF: 11.446)

    2. Hsu, C.-C., Lin, Y.-C., Lin Y.-Y., Lin, Li, H.-T., Ni, C.-S., Liu, C.-I.,  Chang, C.-C., Lin, L.-C., Pan, Y.-T., Liu, S.-F., Liu, T.-Y.#, Chen, H.-Y.# (2022) Trapa natans husk-derived nanoporous carbons as electrode materials for sustainable high-power microbial fuel cell-supercapacitor systems, Advanced Energy and Sustainability Research, 2100163. (#corresponding author; SCI) 獲選期刊當期封面 
    3. Liu, Y.-C.*, Hung, Y.-H.*, Sutarsis, Hsu, C.-C., Ni, C.-S., Liu, T.-Y.#, Chang, J.-K.#, Chen, H.-Y.# (2021), Effects of surface functional groups of coal-tar-pitch-derived nanoporous carbon anodes on microbial fuel cell performance", Renewable Energy, 171, 87 - 94 (*equal contribution; #corresponding author; SCI, 2021 IF: 8.634)
    4. Liu, T.-Y. (2021) Using tripartite split-sfGFP for the study of membrane protein-protein interactions. In: Sanchez-Serrano J.J., Salinas J. (eds) Arabidopsis Protocols. Methods in Molecular Biology, vol 2200. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0880-7_15
    5. Liu, Y.-C., Hung, Y.-H., Liu, S.-F., Guo, C.-H., Liu, T.-Y.#,  Sun, C.-L.#, and Chen, H.-Y.# (2020) Core-shell structured multiwall carbon nanotube–graphene oxide nanoribbon and its N-doped variant as anodes for high-power microbial fuel cells, Sustainable Energy & Fuels, 4, 5339 - 5351 (#corresponding author; SCI, 2021 IF: 6.367)
    6. Hung, Y.-H., Liu, T.-Y.#, and Chen, H.-Y.# (2019) Renewable coffee-waste-derived porous carbons as anode materials for high-performance sustainable microbial fuel cells, ACS Sustainable Chemistry & Engineering, 7, (20) 16991-16999 (#corresponding author; SCI, 2020 IF: 8.198)
    7. Huang, L.-K., Liao, Y.-Y., Lin, W.-H., Lin, S.-M., Liu, T.-Y., Lee, C.-H., and Pan, R.-L., (2019) Potassium stimulation of IAA transport mediated by the Arabidopsis importer AUX1 investigated in a heterologous yeast system. Journal of Membrane Biology 252(2-3):183-194 (SCI, 5-year IF: 1.877)
    8. Liu, T.-Y.#, Chou, W.-C., Chen, W.-Y., Chu, C.-Y., Dai, C.-Y., and Wu, P.-Y. (2018) Detection of membrane protein-protein interaction in planta based on dual-intein-coupled tripartite split-GFP association. The Plant Journal 94(3):426-438. (#corresponding author; SCI, 5-year IF: 6.371) 2018 台灣植物學會新秀獎
    9. Hsu, Y.-D., Huang, Y.-F., Pan, Y.-J., Huang, L.-K., Liao, Y.-Y., Lin, W.-H., Liu, T.-Y., Lee, C.-H., and Pan R.-L. (2018). Regulation of H+-pyrophosphatase by 14-3-3 Proteins from Arabidopsis thaliana. Journal of Membrane Biology 251(2):263-27. (SCI, 5-year IF: 1.877)
    10. Liu, T.-Y.*, Huang, T.-K.*, Yang, S.-Y.*, Hong, Y.-T., Huang, S.-M., Wang, F.-N., Chiang, S.-F., Tsai, S.-Y., Lu, W.-C., and Chiou, T.-J. (2016). Identification of plant vacuolar transporters mediating phosphate storage. Nature Communications 7:11095 (*equal contribution; SCI, 5-year IF: 13.092) The 9th楊祥發院士傑出農業科學年輕學者
    11. Liu, T.-Y., Lin, W.-Y., Huang, T.-K., and Chiou, T.-J. (2014). MicroRNA-mediated surveillance of phosphate transporter on the move. Trends in Plant Science 19, 647-655. (SCI, 5-year IF:13.442)
    12. Huang, T.-K., Han, C.-L., Lin, S.-I, Chen, Y.-J., Tsai, Y.-C., Chen, Y-R., Chen, J.-W., Lin, W.-Y., Chen, P.-M., Liu, T.-Y., Chen, Y.-S., Sun, C-M., and Chiou, T.-J. (2013). Identification of downstream components of PHO2 by quantitative membrane proteomics in Arabidopsis. Plant Cell 25, 4044-4060. (SCI, 5-year IF: 9.996)
    13. Liu, T.-Y., Huang, T.-K., Tseng, C.-Y., Lai, Y.-S., Lin, S.-I, Lin, W.-Y., Chen, J.-W., and Chiou, T.-J. (2012). PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis. Plant Cell 24, 2168-2183. (SCI, 5-year IF: 9.996) 國科會101年度博士後研究人員學術著作獎
    14. Liu, T.-Y., Aung, K., Tseng, C.-Y., Chang, T.-Y., Cheng, Y.-S., and Chiou, T.-J. (2011). Vacuolar Ca2+/H+ transport activity is required for systemic phosphate homeostasis involving shoot-to-root phosphate signaling in Arabidopsis. Plant Physiology 156, 1176-1189. (SCI, 5-year IF: 7.428)
    15. Dettmer, J.*, Liu, T.-Y.*, and Schumacher, K. (2010). Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms. European Journal of Cell Biology 89, 152-156. (*equal contribution; SCI, 5-year IF: 3.753)
    16. Liu, T.-Y., Chang, C.-Y., and Chiou, T.-J. (2009). The long-distance signaling of mineral macronutrients. Current Opinion in Plant Biology 12, 312-319. (SCI, 5-year IF: 7.875)
    17. Neubert, C., Graham, L.A., Black-Maier, E.W., Coonrod, E.M., Liu, T.-Y., Stierhof, Y.-D., Seidel, T., Stevens, T.H., and Schumacher, K. (2008). Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic 9, 1618-1628. (SCI, 5-year IF: 3.989)
    18. Brüx, A.*, Liu, T.-Y.*, Krebs, M., Stierhof, Y.-D., Lohmann, J.U., Miersch, O., Wasternack, C., and Schumacher, K. (2008). Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth inhibition in Arabidopsis. Plant Cell 20, 1088-1100. (*equal contribution; SCI, 5-year IF: 9.996)














    2014 - 迄今 清大生科院豐盛生命師生團契輔導老師

    2016 - 迄今 清交研究生必勝研習會召集人及講員

    2017 清大教師社群“勇於教學”召集人

    2019 清大教師社群“挺身而教”教師社群召集人

    2021 清大教師社群"教師的呼召"召集人

    Undergraduate Research Project

    2015- 2016 科技部大專生專題研究計畫 (林柏成同學)

    2015- 2016 清大生科院大專生專題研究計畫 (吳佩諭同學)

    2016- 2017 科技部大專生專題研究計畫 (戴禎逸同學/榮獲105年度科技部大專學生研究計畫研究創作獎)

    2017- 2018 科技部大專生專題研究計畫 (龍惠方同學)

    2018- 2019 科技部大專生專題研究計畫 (楊承翰同學)

    2018- 2019 清大生科院大專生專題研究計畫 (林桔安同學)

    2019- 2020 科技部大專生專題研究計畫 (堯景泓同學)

    2020- 2021 科技部大專生專題研究計畫 (蔡政達同學)

    2020- 2021 清大生科院大專生專題研究計畫 (黃柏睿同學)

    Grant support

    2014- 2016 科技部生命科學研究發展司

    2016- 2019 科技部自然科學及永續研究發展司

    2019- 2022 科技部生命科學研究發展司

  • 實驗室成員





















    王之怡 蔡明軒




    2019.01.24 年終聚餐

    2020.01.17 年終聚餐

    2020.08.28-29 集集出遊

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